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1973, D.M. Prescott, J.B. Kirkpatrick, “Mass Enucleation of Captured Animal Cells”, in David M. Prescott, editors, Methods in Cell Biology, Volume VII, →ISBN, page 197:
By 12 hours after enucleation, the rate of incorporation of 3H-labeled amino acids is severely reduced, and by 18 hours many enucleates no longer show detectable incorporation.