protospacer
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English
Etymology
Noun
protospacer (plural protospacers)
- (genetics) A particular DNA motif.
- 2015 July 21, Noelia Lander et al., “CRISPR/Cas9-Induced Disruption of Paraflagellar Rod Protein 1 and 2 Genes in Trypanosoma cruzi Reveals Their Role in Flagellar Attachment”, in mBio, volume 6, :
- Genomic DNA was extracted from parental and mutant cell lines and used as the template to amplify fragments of 200 to 1,500 bp containing the protospacer regions chosen for Cas9 targeting of the TcPFR1, TcPFR2, and TcGP72 genes.