retrotranscribe

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English

Etymology

From retro- +‎ transcribe.

Verb

retrotranscribe (third-person singular simple present retrotranscribes, present participle retrotranscribing, simple past and past participle retrotranscribed)

  1. (genetics) To transcribe RNA to DNA.
    • 2015 July 11, “Growth Arrest Specific 1 ( Gas1 ) Gene Overexpression in Liver Reduces the In Vivo Progression of Murine Hepatocellular Carcinoma and Partially Restores Gene Expression Levels”, in PLOS ONE, →DOI:
      One μg of total RNA was retrotranscribed to cDNA using Superscript II RNase H - (Invitrogen), following the manufacturer’s instructions, with random hexamers to prime the elongation reaction.