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2006 November 7, Hideaki Sorachi, “第百二十八訓 運に身分は関係ない [Lesson 128: Luck Has Nothing to Do with Social Status]”, in 銀魂 [Silver Soul], volume 15 (fiction), Tokyo: Shueisha, →ISBN:
…銀サン 今…上様ってきこえませんでした
…Gin-san ima…Ue-sama tte kikoemasen deshita
…Gin-san, just now… I think I heard him say Ue-sama
きこえたな…いや でもまさか ねェ 今 上様って…
Kikoeta na…Iya demo masaka Nē ima Ue-sama tte…
I heard it too… But it can’t be. Hey, did he just say Ue-sama…
2005 December 7, Hideaki Sorachi, “第八十一訓 扇風機つけっぱなしで寝ちゃうとお腹こわしちゃうから気を付けて [Lesson 81: Be Careful When Sleeping with the Fan on or You’ll Get a Stomachache]”, in 銀魂 [Silver Soul], volume 15 (fiction), Tokyo: Shueisha, →ISBN:
Compound of 上(jō, “top-class, the best”) + 様(sama, honorific suffix).[1][2]
Likely a shift from uesama, replacing the 訓読み(kun'yomi) of ue with the 音読み(on'yomi) of jō. Possibly influenced by homophone嬢様(jōsama, “princess”, honorific term of reference for someone else's daughter).